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AGO2 Antibody, FITC conjugated

https://www.academycancerimmunology.org/web/image/product.template/2801/image_1920?unique=b611535
Size : 50 µg Clone Number: Aliases: Ago 2 antibody; AGO2_HUMAN antibody; Argonaute 2 antibody; argonaute 2; RISC catalytic component antibody; Argonaute RISC catalytic component 2 antibody; Argonaute2 antibody; CTA-204B4.6 antibody; dAgo2 antibody; eIF 2C 2 antibody; eIF-2C 2 antibody; eIF2C 2 antibody; Eif2c2 antibody; Eukaryotic translation initiation factor 2C 2 antibody; Eukaryotic translation initiation factor 2C subunit 2 antibody; hAgo2 antibody; MGC3183 antibody; PAZ Piwi domain protein antibody; PPD antibody; Protein argonaute-2 antibody; Protein slicer antibody; Q10 antibody; Slicer protein antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: Q9UKV8 Immunogen: Recombinant Human Protein argonaute-2 protein (517-818AA) Host: Rabbit Reactivity: Human Tested Applications: Background: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The minimal RISC appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions. Clonality: Polyclonal Isotype: IgG Purification Method: 95%, Protein G purified Conjugate: FITC Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 Form: Liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: AGO2 Research Areas: Epigenetics and Nuclear Signaling

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Internal Reference: CSB-PA891731LC01HU