AKT1S1 Antibody

Size : 50 µg Clone Number: Aliases: 40 kDa proline rich AKT substrate antibody; 40 kDa proline-rich AKT substrate antibody; AKT1 S1 antibody; AKT1 substrate 1 (proline rich) antibody; AKT1 substrate 1 antibody; AKT1S 1 antibody; AKT1S1 antibody; AKTS1_HUMAN antibody; Lobe antibody; MGC2865 antibody; PRAS 40 antibody; PRAS antibody; PRAS40 antibody; Proline rich akt substrate antibody; Proline rich Akt substrate 40 kDa antibody; Proline-rich AKT1 substrate 1 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: Q96B36 Immunogen: Recombinant Human Proline-rich AKT1 substrate 1 protein (52-101AA) Host: Rabbit Reactivity: Human, Rat Tested Applications: ELISA, WB, IHC; Recommended dilution: WB: 1: 200-1: 3000, IHC: 1: 20-1: 200 Background: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at \\\'Thr-389\\\', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. Clonality: Polyclonal Isotype: IgG Purification Method: 95%, Protein G purified Conjugate: Non-conjugated Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 Form: Liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: AKT1S1 Research Areas: Neuroscience; Cell biology; Signal transduction