AKT1 (Ab-473) Antibody

Size : 100 µL Clone Number: Aliases: AKT 1 antibody; AKT antibody; AKT1 antibody; AKT1_HUMAN antibody; C AKT antibody; cAKT antibody; MGC99656 antibody; PKB alpha antibody; PKB antibody; PKB-ALPHA antibody; PRKBA antibody; Protein Kinase B Alpha antibody; Protein kinase B antibody; Proto-oncogene c-Akt antibody; RAC Alpha antibody; RAC antibody; Rac protein kinase alpha antibody; RAC Serine/Threonine Protein Kinase antibody; RAC-alpha serine/threonine-protein kinase antibody; RAC-PK-alpha antibody; v akt murine thymoma viral oncogene homolog 1 antibody; vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P31749 Immunogen: Peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt. Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, WB, IHC; WB: 1: 500-1: 1000, IHC: 1: 50-1: 200 Background: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. Baudhuin LM, et al. (2004) FASEB J Feb; 18(2): 341-3. Min YH, et al. (2004) Cancer Res; 64(15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279(34): 35510-7. Ayala G, et al. (2004) Clin Cancer Res; 10(19): 6572-8. Lungu AO, et al. (2004) J Biol Chem; 279(47): 48794-800. Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjugate: Non-conjugated Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: AKT1 Research Areas: Epigenetics and Nuclear Signaling?Neuroscience?Cancer;Metabolism;Signal transduction