AKT1/AKT2/AKT3 (Ab-315/316/312) Antibody

Size : 100 µL Clone Number: Aliases: RAC-PK-alpha; Protein kinase B; Product Type: Polyclonal Antibody Immunogen Species: Homo sapiens (Human) UniProt ID: P31749/P31751/Q9Y243 Immunogen: Peptide sequence around aa.313~317/314~318/310~314 (P-E-Y-L-A) derived from Human AKT1/AKT2/AKT3. Host: Rabbit Reactivity: Human, Mouse, Rat Tested Applications: ELISA, IHC; IHC: 1: 50-1: 100 Background: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. Nelms K, et al. (1999) Annu Rev Immunol. 17: 701-738. Malabarba M G, et al. (1996) Biochem. J. 319: 865-872. Hou J, et al. (1994) Science. 265: 1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343. Takeda K, et al. (1996) Nature. 380: 627-630. Clonality: Polyclonal Isotype: IgG Purification Method: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. Conjugate: Non-conjugated Buffer: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Form: liquid Stroage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Target Names: AKT1/AKT2/AKT3 Research Areas: Epigenetics and Nuclear Signaling?Neuroscience?Cancer;Metabolism;Signal transduction