Acetyl-Histone H4 (Lys8) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4 GeneID: 121504,554313,8294,8359,8360,8361,8362,8363,8364,8365,8366,8367,8368,8370 Uniprot: P62805 Specificity: Acetyl-Histone H4 (Lys8) Antibody detects endogenous levels of Acetyl-Histone H4 only when acetylated at Lys8. Immunogen: A synthesized peptide derived from human Histone H4 around the acetylation site of Lys8. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. Reactivity: Human,Mouse,Rat,Pig,Bovine Prediction: Pig,Bovine,Chicken,Xenopus Application: WB,IHC,IF/ICC,ELISA(peptide) Dilution: WB 1: 500-1: 2000, IF/ICC 1: 100-1: 500, IHC 1: 50-1: 200, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2844617 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF4353-BP Des# Blocking Peptide: Acetyl-Histone H4 (Lys8) Blocking peptide