Acetyl-NF-kappaB p65 (Lys310) Antibody

Volume: 100 µL Host: Rabbit Clonality: Polyclonal Isotype: IgG GeneName: RELA GeneID: 5970 Uniprot: Q04206 Specificity: Acetyl-NF-kappaB p65 (Lys310) Antibody detects endogenous levels of Acetyl-NF-kappaB p65 only when acetylated at Lys310. Immunogen: A synthesized peptide derived from human NF-kappaB p65 around the acetylation site of Lys310. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl. Reactivity: Human,Mouse,Rat Prediction: Pig,Bovine,Horse,Sheep,Dog Application: WB,IHC,IF/ICC,ELISA(peptide) Dilution: WB: 1: 500-1: 2000, IHC: 1: 50-1: 200, IF/ICC 1: 200, ELISA(peptide) 1: 20000-1: 40000 Conjugate: Unconjugated RRID: AB_2835385 An Introduction to Blocking Peptides: A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site. Cat# Blocking Peptide: AF1017-BP Des# Blocking Peptide: Acetyl-NF-kappaB p65 (Lys310) Blocking peptide